Effects of laser polar-body biopsy on embryo quality

Capsule:
Laser polar-body biopsy for preimplantation genetic diagnosis in IVF treatment is associated with reduced embryo quality.

Authors:
Ishai Levin, M.D., Benny Almog, M.D., Tamar Shwartz, B.Sc., Veronica Gold, M.Sc., Dalit Ben-Yosef, Ph.D., Michal Shaubi, M.D., Ami Amit, M.D., Mira Malcov, Ph.D.
Volume 97, Issue 5, Pages 1085-1088, May 2012

Objective:
To evaluate the effect of laser polar-body biopsy (PBB) for preimplantation genetic diagnosis on embryo quality.

Study design:
Retrospective case-control analysis. The quality of 145 embryos after PBB was compared to 276 embryos of the same group of women without biopsy.

Setting:
University-based tertiary-care medical center.

Patient(s):
Women with inherited genetics disease.

Intervention(s):
Laser PBB of IVF embryos for genetic diagnosis.

Main Outcome Measure(s):
The study and control embryos were compared for fertilization rate, pronuclear grading, and cleavage-stage parameters on days 1, 2, and 3 after oocyte retrieval.

Result(s):
The study embryos demonstrated higher rates of cleavage arrest (3.6% vs. 0.7%), higher rate of significant fragmentation on day 2 (9.5% vs. 3.0%), and lower rate of good cleavage embryos on day 2 (69.1% vs. 78.4%) compared with control embryos. On day 3, the study embryos had lower cleavage rates (six or more blastomeres; 56.5% vs. 74.5%), higher fragmentation (11.7% vs. 3.9%), higher rate of embryos presenting inferior cleavage pattern (57.2% vs. 38.5%), and lower mean blastomere number (5.8 ± 2.1 vs. 6.6 ± 1.9) compared with control embryos.

Conclusion(s):
Polar-body biopsy may have a negative effect on embryo quality.

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